Semi-continuous mass culture of rotifers (Brachionus plicatilis) using an automatic feeder

TitleSemi-continuous mass culture of rotifers (Brachionus plicatilis) using an automatic feeder
Publication TypeJournal Article
Year of Publication2012
AuthorsKostopoulou, V, Vasilakis M, Divanach P
JournalAquaculture Research
Pages91 - 98
KeywordsAquaculture, Feeding, Hatcheries, Live feed

This study describes the rotifer mass culture protocol developed at the Institute of Aquaculture (HCMR) in Crete. Rotifers were cultured semi-continuously at a density of 257.6 ± 2.6ind.mL -1, with a daily dilution rate of 25%. An automatic feeder was used to administer dry feed (Selco Sparkle ®) every 10min. A number of factors were investigated so as to provide an in-depth analysis of the advantages and disadvantages of the two systems, especially when compared with traditional protocols such as batch culture. The use of these two systems resulted in a culture of considerable duration (41 days) and daily yield (0.21 ± 0.01ind.10 6day -1L -1), using exclusively dry feed. Both systems can be easily applied in hatcheries, requiring few modifications on existing protocols and contribute to a stable and predictable production. Further improvements include manipulation of the dilution rate, food ration, culture volume and duration, as well as choice of a phytoplankton-based diet. © 2011 Blackwell Publishing Ltd.


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