New polymorphic microsatellite loci for the Macedonian crested newt, Triturus macedonicus, and cross-priming testing in four other crested newt species

TitleNew polymorphic microsatellite loci for the Macedonian crested newt, Triturus macedonicus, and cross-priming testing in four other crested newt species
Publication TypeJournal Article
Year of Publication2008
AuthorsSotiropoulos, K, Tsaparis D, Eleftherakos K, Kotoulas G, Legakis A, Kasapidis P
JournalMolecular Ecology Resources
Volume8
Issue6
Pages1402 - 1404
Keywordsconservation, crested newt, microsatellites, population genetics, Triturus macedonicus
Abstract

Eleven microsatellites have been characterized for Triturus macedonicus. Nine loci showed different variation patterns in a sample of 40 individuals from a single breeding pond in Zagori province (Greece), with an average number of 4.2 alleles per locus and an expected heterozygosity ranging from 0.1199 to 0.8079. Distinct cross-priming amplification rates were recovered on four additional crested newt species. Two monomorphic T. macedonicus loci were polymorphic in other Triturus species. The microsatellites developed herein could be a useful intraspecific genetic tool to undertake fine-scale population genetic analyses as well as in the study of contact zones between crested newt species. © 2008 The Authors.

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-55149089288&partnerID=40&md5=7581e1a1f0eb2b145bb4003662d8d0ee

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