Effects of in vivo treatment with the dopamine antagonist pimozide and gonadotropin-releasing hormone agonist (GnRHa) on the reproductive axis of Senegalese sole (Solea senegalensis)

TitleEffects of in vivo treatment with the dopamine antagonist pimozide and gonadotropin-releasing hormone agonist (GnRHa) on the reproductive axis of Senegalese sole (Solea senegalensis)
Publication TypeJournal Article
Year of Publication2011
AuthorsGuzmán, JM, Cal R, García-López A, Chereguini O, Kight K, Olmedo M, Sarasquete C, Mylonas CC, Peleteiro JB, Zohar Y, Mañanós EL
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume158
Issue2
Pages235 - 245
KeywordsDopamine, FSH, GnRH, LH, Pimozide, Senegalese sole, Spawning, Spermiation
Abstract

The Senegalese sole (Solea senegalensis) is a flatfish that exhibits severe reproductive dysfunctions in captivity. This study aimed at investigating the existence of a dopamine (DA) inhibitory tone on the reproductive axis of this species. Four groups of Senegalese sole breeders were treated with, saline (controls, CNT), the DA antagonist pimozide (PIM, 5mg kg -1), gonadotropin-releasing hormone agonist (GnRHa, 40μg kg -1) or a combination of PIM + GnRHa (COMB). Effects were evaluated on pituitary GnRH levels (ELISA), pituitary gonadotropin subunit transcript levels (qPCR), plasma levels of sex steroids and vitellogenin (ELISA), gonad development (histology), spermiation and egg production. The GnRHa treatment induced egg release and stimulated testis maturation. In males, PIM did not affect pituitary GnRH content, but enhanced GnRHa-induced pituitary GPα transcripts and modified plasma androgen levels; moreover, PIM stimulated spermatogenesis and milt production, both alone and combined with GnRHa. In females, PIM did not affect pituitary and plasma endocrine parameters and did not affect egg production and fertilization success of the broodstock, either alone or in the combined treatment. In conclusion, data indicated the existence of a DA inhibition in mature males, which would be absent or weakly expressed in females. © 2010 Elsevier Inc.

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