Effects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles

TitleEffects of three preservation methods on the evolution of swimbladder radiographic appearance in sea bass and sea bream juveniles
Publication TypeJournal Article
Year of Publication2000
AuthorsKoumoundouros, G, Divanach P, Savaki A, Kentouri M
JournalAquaculture
Volume182
Issue1-2
Pages17 - 25
KeywordsDicentrarchus labrax, Preservation methods, Radiography, Sparus aurata, Swimbladder
Abstract

Sea bass and sea bream juveniles with functional swimbladder were exposed to three methods of preservation: (a) in 10% phosphate buffered formalin, (b) at 4°C, and (c) at -25°C. They were then X-rayed daily over 10 days to evaluate the impact of these methods on the evolution of the radiographic image. All methods significantly decreased the apparent length and depth of the swimbladder, but with different intensities. Freezing had the least effect (9% and 12% reduction in bream and bass, respectively) and only during the first day of preservation. Four days of formalin fixation led to the total disappearance of this image. Preservation at 4°C led to the autolysis and putrefaction of the internal organs which was responsible for the poor images after three days. The results were discussed in terms of their use for the assessment of Juvenile quality by radiography.

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