Effects of dietary administration of Bacillus probiotics on the non-specific immune responses of tinfoil barb, Barbonymus schwanenfeldii (Cyprinidae)

TitleEffects of dietary administration of Bacillus probiotics on the non-specific immune responses of tinfoil barb, Barbonymus schwanenfeldii (Cyprinidae)
Publication TypeJournal Article
Year of Publication2013
AuthorsEslamloo, K, Akhavan SR, Henry M
JournalActa Ichthyologica et Piscatoria
Volume43
Issue3
Pages211-218
KeywordsBacillus licheniformis, Bacillus subtilis, immunostimulant, innate immunity, Ornamental fish
Abstract

Background. Probiotics are known as immunostimulants as well as growth and survival promoters in fish, but
despite the plethora of studies on the effect of probiotics on the fish non-specific immune responses, very few
information is available for ornemental fish. Thus, this study was carried out to investigate the effects of shortterm
dietary administration of Bacillus probiotics (B. subtilis and B. licheniformis) on the immune responses of
tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854).
Materials and methods. Three experimental diets were prepared by supplementing the diets with different concentrations
of probiotics including 0 (Control), 1 × 104 (T1) and 1 × 13306 (T2) CFU · g–1 diet. Tinfoil barb
weighing 16.20 ± 0.13 g were fed different diets for 15 days. At the end of the experiment, blood samples were
collected in order to estimate the hematological (total protein, albumin, and globulin) and immune (total antiprotease,
plasma peroxidase, and bactericidal activities ) parameters.
Results. The plasma total protein, albumin, and globulin of fish were not affected by dietary probiotics. A sixfold
increase in the plasma total antiprotease activity of fish fed 1 × 106 CFU · g–1 diet was observed. Moreover,
the plasma peroxidase and bactericidal activities of fish significantly increased in T2 treatment compared to control.
On the other hand, plasma natural haemolytic complement and lysozyme activities did not significantly vary
between diets although the highest level of these parameters was observed in the probiotic treatments.
Conclusion. This study showed the ability of dietary probiotics to enhance some innate immune responses of tinfoil
barb especially after a 2-week administration of 1 × 106 CFU · g–1 diet (T2). Further study should focus on
the effect of this dietary bacterial concentration on the fish response to bacterial and parasitical challenges in
order to test its potential protective effect against the common pathogens of tinfoil barb.

DOI10.3750/AIP2013.43.3.05

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