Dynamics of gene expression patterns during early development of the European Sea Bass (Dicentrarchus labrax)

TitleDynamics of gene expression patterns during early development of the European Sea Bass (Dicentrarchus labrax)
Publication TypeJournal Article
Year of Publication2015
AuthorsKaitetzidou, E, Xiang J, Antonopoulou E, Tsigenopoulos CS, Sarropoulou E
JournalPhysiological Genomics
Volume47
Pages158-169
KeywordsAquaculture, Development, Dicentrarchus labrax, European seabass, miRNA, mRNA, RNA-Seq, Transcriptomics
Abstract

Larval and embryonic
stages are the most critical period in the life cycle of marine fish. Key
developmental events occur early in development and are influenced
by external parameters like stress, temperature, salinity, and photoperiodism.
Any failure may cause malformations, developmental delays,
poor growth, and massive mortalities. Advanced understanding of
molecular processes underlying marine larval development may lead
to superior larval rearing conditions. Today, the new sequencing and
bioinformatic methods allow transcriptome screens comprising messenger
(mRNA) and microRNA (miRNA) with the scope of detecting
differential expression for any species of interest. In the present study,
we applied Illumina technology to investigate the transcriptome of
early developmental stages of the European seabass (Dicentrarchus
labrax). The European seabass, in its natural environment, is a
euryhaline species and has shown high adaptation processes in early
life phases. During its embryonic and larval phases the European
seabass lives in a marine environment and as a juvenile it migrates to
coastal zones, estuaries, and lagoons. Investigating the dynamics of
gene expression in its early development may shed light on factors
promoting phenotypic plasticity and may also contribute to the improvement
and advancement of rearing methods of the European
seabass, a species of high economic importance in European and
Mediterranean aquaculture. We present the identification, characterization,
and expression of mRNA and miRNA, comprising paralogous
genes and differentially spliced transcripts from early developmental
stages of the European seabass. We further investigated the detection
of possible interactions of miRNA with mRNA

DOI10.1152/physiolgenomics.00001.2015.

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