Cyto-histological examination of post-vitellogenesis and final oocyte maturation in captive-reared striped bass

TitleCyto-histological examination of post-vitellogenesis and final oocyte maturation in captive-reared striped bass
Publication TypeJournal Article
Year of Publication1997
AuthorsMylonas, CC, Woods III LC, Zohar Y
JournalJournal of Fish Biology
Volume50
Issue1
Pages34 - 49
KeywordsAquaculture, Final oocyte maturation, Morome, Oogenesis, striped bass
Abstract

The ovarian development of captive-reared, striped bass Morone saxatilis was examined during a 10-week period encompassing the spawning season. Vitellogenic oocytes in March had a mean diameter of 838 ± 18 μm and did not grow significantly therafter. Except from one non-hormone-treated fish, all females failed to undergo final oocyte maturation (FOM) and their ovaries became atretic with the onset of high spring temperatures. A clearing fixative was found useful in identifying early of atresia, evident by the absence of the germinal vesicle (GV). Final oocyte maturation of fish maturation of fish treated with gonadotropin-releasing hormone agonist (GnRhA) consisted of two phases. Early FOM lasted from 1 to 3 weeks, and was associated with lipid-droplet coalescence, and displacement of the GV and yolk globules to the peripheral cytoplasm. Late FOM lasted <24 h, and consisted of yolk-globule coalescence and GV breakdown (GVBD). Ovulated eggs had completely coalesced lipid and yolk masses, with cortical alveoli lined against the cell wall. Both phases of FOM were associated with significant increases in oocyte diameter. Striped boss oocytes showed important morphological differences compared to oocytes of other members of the Moronidae family, in terms of percentage lipid content, chorion thickness and degree of hydration after ovulation.

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