Control of the Bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions

TitleControl of the Bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions
Publication TypeJournal Article
Year of Publication2000
AuthorsMakridis, P, Fjellheim AJ, Skjermo J, Vadstein O
JournalAquaculture
Volume185
Issue3-4
Pages207 - 218
KeywordsBioencapsulation, ELISA, Immunocolony blot, Probiotics
Abstract

The accumulation of bacteria in Brachionus plicatilis and Artemia franciscana during a short-term incubation was quantified using immunocolony blot (ICB) and an enzyme-linked immunosorbent assay (ELISA). Four bacterial strains, isolated from turbot and halibut, were grazed effectively by both species when given at high concentrations (≥ 5 X 107 bacteria ml-1). B, plicatilis accumulated 21-63 X 103 bacteria per rotifer and A. franciscana up to 45 X 103 bacteria per metanauplius after 20-60 min of grazing. The composition of the bacterial microflora of the live food organisms changed drastically, as the bioencapsulated strains comprised up to 100% of the total count of colony-forming units. After incubation in the bacterial suspensions, B. plicatilis and A. franciscana were transferred to seawater with added microalgae (Tetraselmis sp., 2 mg C I-1), to evaluate the persistence of the changed bacterial composition in conditions similar to those present in a first feeding tank. The bioencapsulated bacteria decreased in numbers, but in most cases remained present in both live food organisms after 24 h. It is possible, after a short-term incubation, to replace opportunistic (r-selected) bacteria present in the live food cultures with other bacteria, which persist as a dominant part of the bacterial flora of the live food for a relatively long period of time (4-24 h). (C) 2000 Elsevier Science B.V.

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