Comparative study of biochemical and immunological biomarkers in three marine bivalves exposed at a polluted site

TitleComparative study of biochemical and immunological biomarkers in three marine bivalves exposed at a polluted site
Publication TypeJournal Article
Year of Publication2013
AuthorsCotou, E, Tsangaris C, Henry M
JournalEnvironmental Science and Pollution Research
Volume20
Issue3
Pages1812 - 1822
KeywordsBiochemical biomarkers, Bioindicators, Callista chione, Immunomarkers, Mytilus galloprovincialis, Oxidative stress, Pollution assessment, Venus verrucosa
Abstract

A battery of biochemical and immunological biomarkers used for pollution assessment were measured for first time in the clams Venus verrucosa and Callista chione and were compared with those of the mussel Mytilus galloprovincialis, a well-established indicator organism utilized in numerous environmental monitoring programs. Clams and mussel were transplanted at a polluted and a reference site or maintained at the laboratory. Among biochemical biomarkers, acetylcholinesterase did not differ at the polluted site in all species, but there was a significant difference between the mussel and the clams, glutathione S-transferase showed a clear inhibition at the polluted site in all species and a significant difference between the two clams was also indicated, while catalase activities were increased only in V. verrucosa at the polluted site and not in mussel or the other clam. Immunological biomarkers responses were also pronounced at the polluted site. Lysozyme activity was species-dependent whereas respiratory burst activity measured as luminol-dependent chemiluminescence (CL) was site and stimulus dependent, and it was evident in M. galloprovincialis and V. verrucosa and not in C. chione. Further investigation focused on biochemical and immunological biomarkers related with the oxidative mechanisms in clams will strengthen and expand their use as bioindicators for pollution assessment. © 2012 Springer-Verlag.

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84874322576&partnerID=40&md5=b71b2421254049bfb45dd5c2d1c6acd1
DOI10.1007/s11356-012-1150-3

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