Application of controlled-release, GnRHa-delivery systems in commercial production of white bass X striped bass hybrids (sunshine bass), using captive broodstocks

TitleApplication of controlled-release, GnRHa-delivery systems in commercial production of white bass X striped bass hybrids (sunshine bass), using captive broodstocks
Publication TypeJournal Article
Year of Publication1996
AuthorsMylonas, CC, Magnus Y, Gissis A, Klebanov Y, Zohar Y
JournalAquaculture
Volume140
Issue3
Pages265 - 280
KeywordsDomestication, Egg quality, GnRH, Hybrid striped bass, Induced spawning, Moronidae, White bass
Abstract

Hatchery-produced white bass (Morone chrysops) and striped bass (M. saxatilis) reared to maturity in a commercial aquaculture facility, were successfully spawned using controlled-release delivery systems containing the gonadotropin-releasing hormone analog DAla6, Pro9[NEt]-GnRH (GnRHa). Two-year-old white bass females (mean weight, 0.81 kg) were implanted with different polymer-based, GnRHa delivery systems at doses ranging from 40 to 89 μg GnRHa kg-1 body weight. GnRHa treatment on 20 February 1994, when females contained oocytes up to 720 μm in diameter, induced ovulation of all fish between 35 to 82 h after treatment. The white bass eggs produced were fertilized with sperm from striped bass for the production of sunshine bass. An average of 294500 eggs kg-1 were produced, with a mean fertility of 81.2%, 24 h survival of 46.5%, and overall hatching success of 45%. Survival from hatch to 30 days post-hatch was 78% and the fry weighed between 0.07 and 0.1 g. Overripening of eggs began within 1 h from ovulation and maximum fertilization (60%) was observed when eggs were stripped 0.5 h after ovulation. Fertilization success decreased thereafter to 31% and 10% by 1 h and 3 h after ovulation, respectively. Control fish not treated with GnRHa did not show any signs of final oocyte maturation during the period of the study. GnRHa administration via controlled-release delivery systems appears to be a very effective method for inducing high fecundity ovulation of captive white bass broodstocks, and producing eggs of high fertility and hatching success.

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