Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family

TitleAdditional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family
Publication TypeJournal Article
Year of Publication2005
AuthorsBrown, RC, Tsalavouta M, Terzoglou V, Magoulas A, McAndrew BJ
JournalMolecular Ecology Notes
Volume5
Issue3
Pages605 - 607
KeywordsCross-species amplification, Gilthead seabream, microsatellites, Sparidae, Sparus aurata
Abstract

Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species. © 2005 Blackwell Publishing Ltd.

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